Perceptions #1

Perceptions, they can work to your favor, or sometimes the opposite.

It's actually quite scary, when people jump into conclusion without understanding the full story.

Or rather, believing only in what they want to hear.

If you criticize people's work, ask if you can do it better and faster.

When UPLC sensitivity dropped to 2-3 times

I once encountered a case whereby injecting the same amount of standard into 2 UPLCs side by side, the area for UPLC#2 is about 2 - 3 times smaller than UPLC#1.

Customer reported that their %RSD is good, but they aren't sure why the area is so much different.

Well... I was equally baffled too.

1. Performed a leak test and there was no leak from the sample syringe.
2. Lamp energy seems fine.
3. Guessed there could be a clogged somewhere in the needle or along the SM fluidics.
4. Since it was also due for PM, might as well start replacing the needful.

Then I found out.

Someone configured the volume syringe 100uL instead of the rightful 250uL.



Not sure whether to laugh or cry eh...